Profile

Education and Qualifications

2021                            Diplomate,   American Board of Toxicology

2018                            Diplomate, American College of Veterinary Pathologists (Anatomic Pathology)

2012 - 2016                 Ph.D., Doctor of Philosophy in Veterinary Pathology

                                    Western College of Veterinary Medicine

                                    University of Saskatchewan, Canada

2009 - 2012                 M.Sc., Master of Science in Veterinary Pathology

                                    Western College of Veterinary Medicine

                                    University of Saskatchewan, Canada

1996 - 2002                 D.V.M., Doctor of Veterinary Medicine

                                    Faculty of Veterinary Medicine

                                    University of Belgrade, Serbia

Present Position:

April 2017 –                ITR Laboratories Canada Inc.       

Present                        Senior Veterinary Pathologist/Veterinary Pathologist

• Conduct and supervise gross pathological (routine and specialized necropsy techniques) and histopathological examination of laboratory animals (rat, mice, dog, minipig, monkey) in the preclinical GLP and non-GLP acute, subacute, subchronic, and chronic general toxicology oral, intravenous, inhalation, dermal, and carcinogenicity studies.
• Summarize, interpret, and discuss necropsy, histopathology, clinical pathology, clinical chemistry, and hematology results and communicate these results to Study Directors.
• Develop and validate investigative techniques, and interpret results of immunohistochemistry, immunofluorescence, tissue cross reactivity test, electron microscopy and image analysis.
• Write and review pathology reports, research reports and scientific publications related to Toxicologic Pathology.
• Perform Pathology Peer Reviews of internal company’s and external industry’s GLP and non-GLP studies.
• Contribute to Study Directors and Sponsors preparation of study plans with appropriate experimental design, species, dose selection, experimental procedures, and GLP compliance.
• Provide guidance to Study Directors in determination of specific target organs, NOAEL and LOAEL of toxicity based on the overall pathology and other laboratory results and contribute to understanding of test item-related toxicity mechanisms.
• Interact with Sponsors and present pathology results to the public.
• Impact: Completion of over 90 studies as study pathologist and 5 as peer review pathologist.

Previous Employment History:

2016 - 2017                 Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada

Postdoctoral Research Fellow, Department of Large Animal Clinical Sciences

• Support Genome Canada project as Investigative Pathologist in the development of reproductive model of Porcine Reproductive and Respiratory Syndrome (PRRS) virus infection (gilts and fetuses) conducting necropsies, histopathology, tissue distribution, immunochemistry, image analyses, data collection, statistical analyses, grants, protocols, and research papers writing, presentations and management.

2016-2017                   Prairie Diagnostic Services, Inc., Saskatoon, Canada

Veterinary Pathologist (Veterinary diagnostic service)

• Conduct necropsy and histopathological examinations of tissues derived from all species of livestock, wildlife, laboratory, and pet animals.
• Consult veterinarians and producers interpreting and reporting lesions and recommending ancillary testing to the clients.

2009 - 2016                 Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada

Veterinary (Anatomic) Pathologist (in training)

• D. thesis title: Pathologic Evaluation of Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection of Pregnant Gilts during Late Gestation.

• MSc thesis title: Porcine Milk Fat Globule Membrane Proteins that Bind to F4ac Fimbriae and prevent attachment of Escherichia coli to enterocytes.

2008 - 2009                 Scientific Institute of Veterinary Medicine of Serbia (NIVS), Belgrade, Serbia

Head, Department for Receiving Material, Washing, and Sterilization

• Manage organization of sampling, receiving, and preparation of the biological materials and perform daily communication with clients, industry, and veterinary control officers.

2002 - 2008                 Scientific Institute of Veterinary Medicine of Serbia (NIVS), Belgrade, Serbia

Research Fellow, Department for Animal Nutrition and Feed Quality Control, Laboratory for Bacteriology and Mycology

• Conduct diagnostic microbiology tests for detection of feed-borne pathogens.
• Introduce novel laboratory protocols (ELISA, PCR) for the detection of animal pathogens and mycotoxins.
• Investigate pathogenesis of Fusarium spp. associated toxicities detecting gross pathological lesions and identifying mycotoxin producing subspecies of molds in the feed.
• Write and prepare multiple scientific journal papers (in Serbian) and dozens oral presentations mostly about mycotoxicoses in animals and their danger for a human health.
• Impact: conducted over 200 pig necropsies and developed and validated EIA assays for quantification of Fumonisin B1, Zearalenone, DON, and T-2 in feed material and animal tissue.

Professional activities and achievements:

• Technical skills:

• Diagnostic and Toxicologic pathology: necropsy, histopathology, immunohistochemistry, immunofluorescence, TUNEL
• Development of cellular immune assays: isolation of PBMC, FACS analysis, Flow cytometry staining, MAC’s cell sorting, ELISA, ELI Spot
• Cell culture: Culture of PBMCs, IPEC-J2
• Molecular biology: PCR, quantitative real-time PCR, cloning
• Proteomics: SDS-PAGE and 2D-electrophoresis, Electro-elution, Western blot, Size Exclusion, Affinity Chromatography, Surface Plasmon Resonance
• Conventional techniques in diagnostic bacteriology, mycology, and serology
• Good Laboratory Practices (GLP)

• Computer skills:

• Pathology and histopathology software (Provantis)
• Digital Imaging: OlyVIA, Image-Pro Plus, Image-Pro Premier, Aperio ImageScope
• Statistics: Stata, SPSS, Prism
• Microsoft Office - Word, PowerPoint, Excel
• Easy and fast mastering of new software

• Languages: English, Serbian – actively; French – passively

• Membership:

• American College of Veterinary Pathologists
• Society of Toxicologic Pathology
• Canadian Veterinary Medical Association
• Saskatchewan Veterinary Medical Association
• Charles Louis Davis Foundation for the Advancement of Veterinary and Comparative Pathology

• Scholarships/Awards:

• David A. Benfield Travel Fellowship Award (2014)
• Saskatchewan Innovation and Opportunity Scholarship (2014)
• University of Saskatchewan Graduate Scholarship (2012-2015)
• Saskatchewan Innovation and Opportunity Scholarship (2012)
• Vétoquinol Epidemiology/Food Safety Award (2011)
• Graduate Research Fellowship (2011-2012)
• University of Saskatchewan Graduate Scholarship (2009-2011)
• Kingdom of Norway Government's scholarship (2001)

CONTINUING EDUCATION:

STP 38th Annual Symposium, Raleigh, North Carolina, June 22–27, 2019: Environmental Toxicologic Pathology and One Health

CE Course: Medical Device Safety Assessment: The Frontiers of Safety Assessment Pathology

CE Course: Otic Toxicologic Pathology

ITR Laboratories Canada Inc.: 2017-2020 GLP certification training

CL Davis Foundation: 2015 Descriptive Veterinary Pathology Course

Canadian Association of Veterinary Pathologists: 2015 Forensic Pathology Course

University of Saskatchewan, Workplace Safety and Environmental Protection: Biosafety Course, Laboratory Safety Course

University of Saskatchewan, Canadian Council on Animal Care: Experimental Animal user Training Course for Farm Animals

PUBLICATION RECORD – MANUSCRIPTS

• Malgarin, C. M., Zarate, J. B., Novakovic, P., Detmer, S. E., MacPhee, D. J., Harding, J. C. S. Samples sizes required to accurately quantify viral load and histological lesion severity at the maternal-fetal interface of PRRSV inoculated pregnant gilts. Journal of Veterinary Diagnostic Investigation. 2021 Jan 15.
• Malgarin, C. M., Nosach, R., Novakovic, P., Suleman, M., Ladinig, A., Detmer, S. E., MacPhee, D. J., Harding, J. C. S. Classification of fetal resilience to porcine reproductive and respiratory syndrome (PRRS) based on temporal viral load in late gestation maternal tissues and fetuses. Virus Research, Volume 260, 15 January 2019.
• Suleman, M., Novakovic, P., Malgarin, C. M., Detmer, S. E., Harding, J. C. S., MacPhee, D. J., Spatiotemporal immunofluorescent evaluation of porcine reproductive and respiratory syndrome virus transmission across the maternal-fetal interface. Pathogens and Disease, Volume 76, Issue 5, 1 July 2018.
• Novakovic, P., Detmer, S. E., Suleman, M., Malgarin, C. M., MacPhee, D. J., Harding, J.C.S (2018) Histopathological changes associated with placental separation in porcine reproductive and respiratory syndrome infected gilts. Veterinary Pathology, March 22, 2018.
• Novakovic, P., Harding, J. C. S., Al-Dissi, A. N., Detmer, S. E. (2017) Type 2 porcine reproductive and respiratory syndrome virus infection increases apoptosis at the maternal-fetal interface in late gestation pregnant gilts. PLoS ONE 12(3): e0173360.
• Harding, J. C. S., Ladinig, A., Novakovic, P., Detmer, S. E., Wilkinson, J. M., Yang, T., Lunney, J.K., Plastow, G. S.: Novel insights into host responses and reproductive pathophysiology of porcine reproductive and respiratory syndrome caused by PRRSV-2. Veterinary Microbiology, March 2017.
• Novakovic, P., Harding, J. C., Ladinig, A., Al-Dissi, A. N., MacPhee, D. J., Detmer, S. E.: Relationships of CD163 and CD169 positive cell numbers in the endometrium and fetal placenta with type 2 PRRSV RNA concentration in fetal thymus. Veterinary Research, 47, 2016, 76.
• Novakovic, P., Harding, J. C. S., Al-Dissi, A. N., Ladinig, A., Detmer, S. E.: (2016) Pathologic Evaluation of Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection at the Maternal-Fetal Interface of Late Gestation Pregnant Gilts. PLoS ONE, 11, 2016, e0151198.
• Novakovic, P., Charavaryamath, C, Moshynskyy, I., Lockerbie, B., Kaushik, R. S., Loewen, M. E., Kidney, B. A., Stuart, C., Simko, E.: Evaluation of inhibition of F4ac positive Escherichia coli attachment with xanthine dehydrogenase, butyrophilin, lactadherin and fatty acid binding protein; BMC. Veterinary Research, 2015, 11, 238.
• Novakovic, P., Huang, Y., Lockerbie, B., Shahriar, F., Kelly, J., Gordon, J., Middleton, D., Loewen, M., Kidney, B., Simko, E.: Identification of F4ac-binding proteins of porcine milk fat globule membrane. Canadian Journal of Veterinary Research, 79, 2015, 120–128.

PUBLICATION RECORD – ARTICLES IN CONFERENCE PROCEEDINGS AND CONFERENCE PRESENTATIONS/ABSTRACTS

• Novakovic P., Detmer S. E., Suleman M., Malgarin C. M., Ladinig A., MacPhee D., Harding J. C. S.: Microscopic assessment of fetal placental separations during early type 2 PRRS virus infection in late pregnant gilts. Advances in Pork Production (2017) Volume 28, 2017 Banff Pork Seminar.
• Novakovic, P., Detmer, S.E., Muhammad, S., Malgarin, C. M., Ladinig, A., MacPhee, D. J., Harding, J. C. S.: Pathological findings at the maternal‐fetal interface during the early type 2 PRRS virus infection of late gestation pregnant gilts. North American PRRS Symposium, Chicago, USA, December 3-4, 2016.
• Novakovic, P., Al-Dissi, A., Harding, J. C. S., Detmer, S. E.: Relationship between vasculitis, endometrial inflammation and cell death at maternal-fetal interface of type 2 PRRSV infected pregnant gilts. International Pig Veterinary Society Congress, Dublin, Ireland, June 7-10, 2016.
• Harding J. C. S., Ladinig A., Novakovic P., Detmer S. E., Wilkinson J. M., Yang T., Lunney J. K., Plastow G. S.: 2016. PRRSV and the pregnant female. Adv. Pork Prod. Vol 27 (Proc Banff Pork Seminar)
• Novakovic, P., Harding, J. C. S., Detmer, S. E.: Detection of type 2 PRRS virus in the fetal implantation sites of pregnant gilts. XVII International Symposium of the World Association of Veterinary Laboratory Diagnosticians, Saskatoon, Canada, June 15-18, 2015.
• Novakovic, P., Harding, J. C. S., Detmer, S. E.: CD163 and CD169 markers at the maternal-fetal interface during type 2 PRRSV infection. International PRRS Congress, Ghent, Belgium, June 3-5, 2015.
• Novakovic, P., Harding, J. C. S., Detmer, S. E.: Apoptosis at the maternal-fetal interface of type 2 PRRSV infected pregnant gilts. International PRRS Congress, Ghent, Belgium, June 3-5, 2015.
• Novakovic, P., Harding, J. C. S., Detmer, S. E.: Relationship of CD163 and CD169 positive macrophages in type 2 PRRSV infected maternal-fetal interface. North American PRRS Symposium, Chicago, USA, December 5-6, 2014.
• Novakovic, P., Al-Dissi, A., Ladinig, A., Harding, J., Detmer, S.: Relationship of the microscopic lesions and viral load in the fetal implantation sites for type 2 PRRS virus infected pregnant gilts. International Pig Veterinary Society Congress, Cancún, México, June 8-11, 2014.
• Novakovic, P., Al-Dissi, A., Ladinig, A., Harding, J., Detmer, S.: Relationship of the microscopic lesions and viral load in the fetal implantation sites for type 2 PRRS virus infected pregnant gilts. American Association of Swine Veterinarians Annual Meeting, Dallas, USA, March 1-4, 2014.
• Novakovic, P., Yanyun, H., Charavaryamath, C., Lockerbie, B., Kelly, J., Loewen, M. E., Simko, E.: Porcine milk fat globule membrane proteins that bind to F4ac fimbria of Escherichia coli. 2012 Experimental Biology Conference, San Diego, USA, April, 2012.