These tissues were part of a study examining the pulmonary toxicity and carcinogenicity of inhaled diesel exhaust in F344 rats (Mauderly 1987).
Acrylamide was used to induce lesions for positive-control neuropathology in accordance with the EPA’s Tier 2 regulations on toxicology of engine emissions from testing fuels and fuel additives (40 CFR, Part 79.66(e)(3)).
Rats were administered Compound X or Compound Y daily by the oral gavage route for 10 days. Animals sacrificed on Day 5 and Day 10.
Calves developed clinical signs of weakness, ataxia, depression, dehydration, diarrhea, and loss of appetite; Four died. A white powdery substance was found nearby.
Groups of female rats, 10-11 weeks old, were exposed to a toxic vapor 6 hrs/day, 5 days/week for 6 weeks. The rats were then observed for 18 months after the end of exposure.