Adult female mixed breed pony

SIGNALMENT: (age, breed, sex, species, and scientific name) 
Adult female mixed breed pony

TREATMENT PROTOCOL: (including test  compound)
3-methylindole, 100 mg/kg body weight, per os, 5 days before necropsy

GROSS PATHOLOGY
No gross nasal lesion. Lungs overinflated. 

LABORATORY RESULTS: (clinical pathology, microbiology, etc…) 

MORPHOLOGIC DIAGNOSI(E)S AND ETIOLOGY: 

Olfactory mucosal epithelial and Bowman’s gland epithelial hyperplasia; Response to injury by 3-methylindole 

CONTRIBUTOR'S COMMENTS: 

3-methylindole-induced olfactory mucosal damage is less severe than bronchiolar epithelial damage in ponies.  In contrast, mice suffer severe olfactory mucosal damage with metaplasia and scarring, whereas bronchiolar epithelial damage is milder and transient.  

IMAGES: 

Legend:  4x objective, HE.  Mild mucosal injury in ethmoid turbinate.  (Objective of the microscope or the magnification of the Electron Microscope when each photograph is taken, type of stain used, and descriptive legend you would suggest for the submitted image.)  

Legend:  20 x objective, HE.  Uneven mucosal surface, hypertrophy and mitotic figures in mucosal and Bowman’s glandular epithelial cells.  (Objective of the microscope or the magnification of the Electron Microscope when each photograph is taken, type of stain used, and descriptive legend you would suggest for the submitted image.)  

Legend:  40x objective, HE.  Epithelial hypertrophy and mitotic figures in mucosal epithelium and in Bowman’s glands.  (Objective of the microscope or the magnification of the Electron Microscope when each photograph is taken, type of stain used, and descriptive legend you would suggest for the submitted image.) 

Legend:  40x objective, IHC for PCNA with hematoxylin counterstain, DAB chromagen.  Epithelial nuclei, especially those in Bowman’s glands, are labeled by PCNA.  (Objective of the microscope or the magnification of the Electron Microscope when each photograph is taken, type of stain used, and descriptive legend you would suggest for the submitted image.) 

 

r134a.jpg

4x H&E

r134b.jpg

20x H&E. 

r134c.jpg

40x H&E

r134ds.jpg

40x, PCNA IHC

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