Preparation of Three-dimensional (3-D) Human Liver (HepaRG) Cultures for Histochemical and Immunohistochemical Staining and Light Microscopic Evaluation
The latest Toxicologic Pathology Journal podcast is available now! Listen to an engaging discussion between our Podcast Manager, Dr. Liz McInnes (Syngenta), and Dr. Darlene Dixon (NTP/NIEHS). The subject is Dr. Dixon’s latest publication on the preparation of three-dimensional (3-D) human liver (HepaRG) cultures for histochemical and immunohistochemical staining and light microscopic evaluation. Dr. Dixon begins by detailing what other tissues have been explored as 3-D spheroids and then lists the differences between her group’s technique and conventional methods such as the Matrigel sandwich technique. Dr. Dixon explains why her group’s technique allows for better visualization by light microscopy of cells and cellular structures in 3-D cultures, and ends by describing the immunohistochemistry approaches used to characterize the human hepatocyte spheroids.
You can download and listen to the podcast below. You can access the manuscript at this link.